Comparison of biological, biochemical, immunological, and immunochemical techniques for typing herpes simplex virus isolates.

A Preliminary Study on the Molecular Identification of Herpes Simplex Virus Type 1 in Iranian Population

Herpes Simplex Virus Type 1 (HSV-1) is a member of the Herpesviridae family that causes herpetic disease in human. During the last 25-30 years, many investigations have been conducted on pathogenesis and molecular biology of this virus in several countries. In Iran HSV-1 has been isolated from patients and detected using immunological techniques. In this study, we investigated the molecular asp...

Comparison of an avidin-biotin immunoassay with three commercially available immunofluorescence kits for typing of herpes simplex virus.

An avidin-biotin complex system was compared with three commercially available immunofluorescence kits for serotyping herpes simplex virus isolates from clinical specimens. Sensitivity values showed that the Electro-Nucleonics and Immulok reagents were useful in detecting the presence of virus, whereas the predictive values showed that the Syva and Immulok reagents possessed adequate discrimina...

Comparison of a commercial ELISA system with restriction endonuclease analysis for typing herpes simplex virus.

Isolates of herpes simplex virus which had previously been typed by restriction enzyme analysis were typed again with a commercial ELISA system using polyclonal antibodies. There was complete correlation between the two techniques. Although restriction is more precise and definitive, when typing only is required the simplicity of ELISA makes it the preferred technique.

Rapid typing of herpes simplex virus strains using the indirect immunoperoxidase method.

The indirect immunoperoxidase technique with type-specific antisera was compared to kinetic neutralization for the typing of herpes simplex virus strains. There was complete agreement between the results obtained by kinetic neutralization and indirect immunoperoxidase. The indirect immunoperoxidase assay was far simpler to perform and interpret than the kinetic neutralization tests, and it offe...

Restriction Enzyme Analysis of Thymidine Kinase Gene in Four Iranian Isolates of Herpes Simplex Virus Type 1